The Conference is composed of delegations from the relevant committees of the national parliaments of EU members states and the European Parliament. They usually comprise members of the budget, economic, social and European affairs committees. The composition and size of delegations are determined by each parliament independently. Moreover, each national parliament of a candidate country may be represented by two observers. The President of the European Council, the President of the Eurogroup and the relevant members of the European Commission are invited to the meetings of the Conference to present the priorities and strategies of the EU.

The Conference operates on the basis of the principle of consensus. In accordance with the rules of procedure, the Presidency parliament may present non-binding conclusions on the outcome of the meeting, in the first semester - together with the European Parliament. No Presidency has presented such conclusions so far.